Hagelauer and Faust: Catalytic activity and activation energy of creatine kinase isoenzymes
نویسنده
چکیده
The catalytic activity and activation energy of an enzyme are obtained by measuring the rate of the enzymic reaction at two different temperatures. With the aid of the Arrhenius equation, these two parameters can be used to calculate a value proportional to the quantity of enzyme. Using this approach to investigate the isoenzymes of creatine kinase, it was shown that the activation energy increased in the order creatine kinase MM, MB, BB. Mixtures of the isoenzymes showed an apparent mean activation energy, which likewise could be determined using the Arrhenius equation.
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